Journal: Cellular and molecular biology (Noisy-le-Grand, France)
Article Title: lncRNA PCAT1 might coordinate ZNF217 to promote CRC adhesion and invasion through regulating MTA2/MTA3/Snai1/E-cadherin signaling.
doi: 10.14715/cmb/2021.67.4.1
Figure Lengend Snippet: Figure 5. The protein expressions of MTA2, MTA3, SNAI1 and E-cadherin in CRC tissue and cell lines. (a) The upregulation of MTA2, MTA3, and SNAI1, and downregulation of E-cadherin in stage 0-I and stage II-III CRC tissues samples. (b) The upregulation of MTA2, MTA3, and SNAI1, and downregulation of E-cadherin in both HT29 and HCT8 cells. * p < 0 05, * p < 0 01 and ***p < 0 001 vs paracancerous CRC or FHC cell line. # p < 0 01, ## p < 0 01, and ### p < 0 001 vs stage 0-I CRC or HT29 cell line.
Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with Rabbit polyclonal MTA2 (1:3000, 17554-1-AP, Proteintech), MTA3 (1:1000, 14682-1-AP, Proteintech), SNAI1 (1:5000, 20874-1- AP, Proteintech), and mouse anti-human E-cadherin (1:2000, 60335-1-Ig, Proteintech) and beta-actin as internal reference (1:8000, 60008-1-Ig, Proteintech).
Techniques: